TRIP Transgenic Rats ImmunoPhenomic

TRIP platform has been created in 1996 and has ever since developed Knock-Out (KO) or Knock-In (KI) rat research models for all (academic or private labs, French or international companies…)

• Gene or large genomic locus function analysis: Gene(s) KO
• SNP studies: KI to induce mutation of interest
• Cellular or protein tracking: reporter KI in frame with your marker protein
• Protein or reporter gene overexpression: KI of your protein of interest or reporter under the control of ubiquitous promoter
• Spatio-temporal control by conditional models
• Human cells or tissues engraftment: immunodeficient models

To discuss your own project or if you are interested in our available research models, please contact Jérôme Jullien and Matthieu Giraud

For a review on rat genome editing and rat models of immune genes and genetic diseases see Chenouard V, Remy S, Tesson L, Ménoret S, Ouisse LH, Cherifi Y, Anegon I. Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models
Front Genet. 2021 Apr 20;12:615491. PMID: 33959146
 

Reporter GFP rats (alive & cryoconserved)

• Remy, S. et al. (2010). New lines of GFP transgenic rats relevant for regenerative medicine and gene therapy. Transgenic Research

 
Immunodeficient rats

• RRG KO (rIL2Rg KO / rRag-1 KO) (cryoconserved)Ménoret, S., et al. (2018). Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models. Transplantation, 102(8), 1271‑1278.
• RRGS (RRG KO with human SIRPα) (alive & cryoconserved)Ménoret, S., et al. 2019. « In Vivo Analysis of Human Immune Responses in Immunodeficient Rats ». Transplantation, novembre. 

Duchenne Muscular Dystrophy  (DMD) disease rat model (alive & cryoconserved)

• Larcher, T., Lafoux, A., Tesson, L., Remy, S., Thepenier, V., François, V., … Huchet, C. (2014). Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PloS One, 9(10), e110371.
• Ouisse, Laure-Hélène, Séverine Remy, Aude Lafoux, Thibaut Larcher, Laurent Tesson, Vanessa Chenouard, Carole Guillonneau, et al. 2019. « Immunophenotype of a Rat Model of Duchenne’s Disease and Demonstration of Improved Muscle Strength After Anti-CD45RC Antibody Treatment ». Frontiers in Immunology 10 (septembre): 2131.
• Caudal, Dorian, Virginie François, Aude Lafoux, Mireille Ledevin, Ignacio Anegon, Caroline Le Guiner, Thibaut Larcher, et Corinne Huchet. 2020. « Characterization of brain dystrophins absence and impact in dystrophin-deficient Dmdmdx rat model ». PLoS ONE 15 (3).

 
Other reporter and conditional models are available in repositories and vendors
Reporter model list
Conditional model list